Journal: Cancers

Article Title: Keratin-14 (KRT14) Positive Leader Cells Mediate Mesothelial Clearance and Invasion by Ovarian Cancer Cells

doi: 10.3390/cancers11091228

Figure Lengend Snippet: ( A ) Mesothelial displacement assays. Ovarian cancer spheroids were generated from wild-type (WT), KRT14 KO and KRT14 OE lines and overlaid onto a confluent layer of mesothelial cells and imaged by light microscope. Representative images following overnight and 48-h co-culture are presented where dotted lines indicate spheroid outgrowth and mesothelial displacement. ( B , C ) Embedding and outgrowth assays. OVCAR4 ovarian cancer spheroids were formed and overlaid into ( B ) Collagen I (24 h) or ( C ) Matrigel (48 h) samples, and subsequently fixed and stained for KRT14 expression. ( D ) Wound-healing assays OVCAR4 cells were grown as a monolayer, cells were wounded, fixed, and stained for KRT14 expression at time-points preceding total wound closure. Images from a representative well are shown with scale bars = 100 µm.

Article Snippet: Spheres were embedded in either 3D Matrigel (354230; BD Biosciences) or rat-tail collagen I (354236; BD Biosciences).

Techniques: Generated, Light Microscopy, Co-Culture Assay, Staining, Expressing